Chinese hamster ovary cells replicate adenovirus deoxyribonucleic acid.
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چکیده
منابع مشابه
Effects of thymidine on deoxyribonucleoside triphosphate pools and deoxyribonucleic acid synthesis in Chinese hamster ovary cells.
Cultured Chinese hamster ovary cells were synchronized by isoleucine starvation. Thymidine was added either during Gl or during S-phase and deoxyribonucleotide pools and the rate of DNA synthesis were measured. Addition of 1 mu thymidine to Gl cells did not appreciably influence the entry of the cells into S-phase but inhibited the rate of DNA synthesis up to 90%. The pools of dTTP, dGTP, and d...
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The neutral amino acid transport systems A, ASC, and L have been characterized in the Chinese hamster ovary cell. System A, defined by its sodium ion dependency and inhibition by 2-methylaminoisobutyric acid, was found to be extremely sensitive to the pH of the external medium and to increase in response to starvation for amino acids. System ASC, identified by its sodium ion dependency and into...
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Proteinase 3(PR3) is a human polymorphonuclear leukocyte serine proteinase and is the main target antigen for antineutrophil cytoplasmic antibodies (ANCA) found in Wegener's granulomatosis (WG). We developed a stable expression system for conformationally intact recombinant PR3 (rPR3) in Chinese hamster ovary cells (CHO-cells). The part of PR3 cDNA that encoded the active form of PR3 was s...
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Epitope tagging is a method of expressing proteins whereby an epitope for a specific monoclonal antibody is fused to a target protein using recombinant DNA techniques. The aim of this study was to sub-clone the peroxisomal protein (PEP) cDNA into a mammalian expression vector leading to the formation of a chimeric PEP-cDNA containing the FLAG epitope. The FLAG-PEP recombinant cDNA was construc...
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Introduction: Heavy ions are nucleus of elements of iron, argon, carbon and neon that all carry positive electrical charges. For these particles to be useful in radiotherapy they need to accelerated to high energy by more than thousand mega volts. Also the cosmic environment is considered to be a complicated mixture of highly energetic photons and heavy ions such as iron. Therefore, the health ...
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ژورنال
عنوان ژورنال: Molecular and Cellular Biology
سال: 1981
ISSN: 0270-7306,1098-5549
DOI: 10.1128/mcb.1.3.208